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Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: All Species
- IMMUNOGEN: Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: CM, Flow, IF, IHC, WB
- APPLICATION NOTE: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
WB: 2-5 ug/ml
Confocal Microscopy
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Properties
- PURIFICATION: Protein G affinity chromatography
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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