BimS/EL/L Antibody [10B12]
CATALOG NUMBER: 36-186- Clonality: Monoclonal
- Tested Applications: ELISA, Flow, ICC, IHC, IP, WB
- Host Species: Rat
- Species Reactivity: Dog, Human, Monkey, Mouse, Rat
- Conjugate: Unconjugated
- Size: 100 ug
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- HOST SPECIES: Rat
- SPECIES REACTIVITY: Dog, Human, Monkey, Mouse, Rat
- IMMUNOGEN: Synthetic peptide corresponding to aa 20-40 of mouse BimL.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, ICC, IHC, IP, WB
- APPLICATION NOTE: ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
- SPECIFICITY: Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.
Properties
- PURIFICATION: >95% (SDS-PAGE)
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a, kappa
- PHYSICAL STATE: Liquid
- BUFFER: Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: Stable for at least 1 year after receipt when stored at -20°C.
Additional Info
- ADDITIONAL NAMES: BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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