Specifications
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
- TESTED APPLICATIONS: IF, IHC, WB
- SET CONTENTS: Bad Antibody, Catalog No. 3343 (50 μg)
Bid Antibody (CT), Catalog No. 3353 (50 μg)
Bik Antibody, Catalog No. 3819 (50 μg)
Bim Antibody (IN), Catalog No. 2065 (50 μg)
Bmf Antibody (NT), Catalog No. 3031 (50 μg)
Hrk Antibody, Catalog No. 3771 (50 μg)
Noxa Antibody, Catalog No. 2437 (50 μg)
PUMA Antibody (CT), Catalog No. 3041 (50 μg)
Properties
- PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. This process is regulated by the interplay of pro- and anti-apoptotic members of the B-cell lymphoma 2 (Bcl-2) family. The BH3-only (Bcl-2-homology domain 3 only) proteins are a pro-apoptotic subgroup of the Bcl-2 family that are critical initiators of lymphocyte apoptosis and other processes. Unlike the other members of the Bcl-2 family which contain at least two Bcl-2 homology (BH) domains, these proteins contain just one, the BH3 domain. Some of the members of this family, such as Hrk, may show little sequence homology to the others, but all BH-only proteins can bind to at least some pro-survival Bcl-2-like proteins through this BH3 domain and trigger apoptosis when overexpressed. For example, the proteins Bad, Bim and Bmf regulate apoptosis by forming heterodimers with the anti-apoptotic proteins Bcl-2 and Bcl-xL. Bim, Noxa, and PUMA are activated by p53 following DNA damage although the exact mechanisms are not entirely clear. Noxa may also be involved with the activation of Bik, a BH3-only protein whose localization in the endoplasmic reticulum (ER) suggests that Bik may play a role in ER-stress-induced apoptosis. While full-length Bid also promotes apoptosis when expressed, its activity is enhanced following cleavage by enzymes such as capsase-8, suggesting that Bid may play a key role in death-receptor induced apoptosis.
For images please see PDF data sheet - 1: Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- 2: Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- 3: Strasser A. The role of BH3-only proteins in the immune systems. Nat. Rev. 2005
- 4: Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.
Set Contents
- Catalog Number 3343: BAD Antibody
- Catalog Number 3353: Bid Antibody
- Catalog Number 3819: Bik Antibody
- Catalog Number 2065: Bim Antibody
- Catalog Number 3031: Bmf Antibody
- Catalog Number 3771: Hrk Antibody
- Catalog Number 2437: Noxa Antibody
- Catalog Number 3041: PUMA Antibody
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
SARS-CoV-2 Omicron BA.1 Variant Pseudovirus
CATALOG NUMBER: 95-201
SARS-CoV-2 WT Pseudovirus
CATALOG NUMBER: 95-200
SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron)
CATALOG NUMBER: 9805