Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (92%)
- IMMUNOGEN: Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1.
The immunogen is located within the last 50 amino acids of Bfl-1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: Bfl-1 antibody can be used for the detection of Bfl-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - SPECIFICITY: At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
- POSITIVE CONTROL 1: Cat. No. 1305 - Human Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1405 - Mouse Kidney Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 1302 - Human Lung Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 19 kDa
Observed: 20 kDa
Properties
- PURIFICATION: Bfl-1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Bfl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BCL2A1
- ADDITIONAL NAMES: Bfl-1 Antibody: GRS, BFL1, ACC-1, ACC-2, HBPA1, BCL2L5, GRS, Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5
- Protein Accession Number: NP_004040
- PROTEIN GI NUMBER: 4757840
- NCBI GENE ID NUMBER: 597
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.
- 1: Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- 2: Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.
- 3: Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.
- 4: Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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