BCL7A Antibody
CATALOG NUMBER: 45-325- Clonality: Polyclonal
- Tested Applications: ELISA, Flow, IF, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
- IMMUNOGEN: The immunogen for this antibody is: C-MKLEASQQNSEEM
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Lymph Node lysates (calculated MW of 25.0kDa according to NP_066273.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1).
- POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: BCL7A
- ADDITIONAL NAMES: BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7
- Protein Accession Number: NP_066273.1,NP_001019979.1
- PROTEIN GI NUMBER: 10337613
- NCBI GENE ID NUMBER: 605
Background
- 1: Zani VJ, Asou N, Jadayel D, Heward JM, Shipley J, Nacheva E, Takasuki K, Catovsky D, Dyer MJ. Molecular cloning of complex chromosomal translocation t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon. Blood 1996 Apr 15;87(8):3124-34
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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