- IAV
- Trial Size
Bcl-2 Antibody
CATALOG NUMBER: 3335- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)
- IMMUNOGEN: Anti-Bcl-2 antibody (3335) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2.
The immunogen is located within the first 50 amino acids of Bcl-2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1-2μg/mL; IHC-P: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples ; Immunofluorescence in human and mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1288 - A20 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1305 - Human Kidney Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 10-401 - Human Kidney Tissue Slide
- POSITIVE CONTROL 4: Cat. No. 1219 – MCF-7 Cell Lysate
- POSITIVE CONTROL 5: Cat. No. 1208 – THP-1 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 26kD
Observed: 26 kD
Advanced Validation
- VALIDATION:
Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain. Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies.
- ISOFORMS: Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). 3337 can detect all isoforms of human, mouse and rat
Properties
- PURIFICATION: Bcl-2 Antibody is Ion exchange chromatography purified.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Bcl-2antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BCL2
- ADDITIONAL NAMES: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
- Protein Accession Number: AAH27258
- PROTEIN GI NUMBER: 20072668
- NCBI GENE ID NUMBER: 596
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
- 1: Lockshin et al. Cell Death Differ. 2000; 7:2-7.
- 2: Cory et al. Oncogene 2003; 22:8590-607.
- 3: Heiser et al. Exp. Geron. 2004; 39:1125-35.
- 4: Hockenbery et al. Nature 1990; 348:334-6.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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