Close Hamburger Menu Close Hamburger Menu
Home / ELISA / BAFF Recombinant Protein
BAFF Recombinant Protein
BAFF Recombinant Protein

BAFF Recombinant Protein

CATALOG NUMBER: 96-827
  • Tested Applications: ELISA, WB

Specifications

  • SPECIES: Human
  • SOURCE SPECIES: HEK293 cells
  • RECOMBINANT PROTEIN SEQUENCE: Ala 134 - Leu 285
  • FUSION TAG: His Tag
  • TESTED APPLICATIONS: ELISA, WB
  • APPLICATION NOTE: This recombinant protein can be used for E, WB. For research use only.
  • PREDICTED MOLECULAR WEIGHT: 18.4 kDa
  • BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human BAFF, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human BCMA, Fc Tag with a linear range of 3.9-15.6 ng/mL.

Properties

  • PURITY: >95% as determined by SDS-PAGE.

    Endotoxin level is less than 1.0 EU per ug by the LAL method.
  • PHYSICAL STATE: Lyophilized
  • BUFFER: PBS, pH7.4
  • STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: BAFF
  • ADDITIONAL NAMES: TNFSF13B,BAFF,BLYS,CD257,DTL,TALL-1,TALL1,THANK,TNFSF20,ZTNF4
  • Protein Accession Number: AAH20674.1
  • NCBI GENE ID NUMBER: 10673

Background

  • B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. 
  • 1: Oren,D.A., et al., 2002, Nat. Struct. Biol. 9 (4): 288–292.
  • 2: Daridon C, er al.,2008, Autoimmun Rev 7 (4): 267–71.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER: 96-827

    Product Discontinued. Please contact us for alternate product(s).

    Datasheet
    Shipping Info

    $65 Standard Overnight flat rate for as many products shipped within the United States.

    $35 Dry Ice Shipment fee may be required on some items.

    Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

    Customize your order

    Bulk Pricing Available

    Custom Formulations: azide free, endotoxin testing & endotoxin removal.

    Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

    Contact our Customer Care Team for more information

    Custom Antibody Services 

    Speak to one of our custom antibody specialists to discuss your next custom antibody project.

    New & Featured Products
    HIV-1 p24 Antibody [10A2] (biotin)
    HIV-1 p24 Antibody [10A2] (biotin)

    CATALOG NUMBER: PM-7371-biotin

    HIV-1 p24 Antibody [9D2F6] (biotin)
    HIV-1 p24 Antibody [9D2F6] (biotin)

    CATALOG NUMBER: PM-7369-biotin

    HIV-1 p24 Antibody [8D7E11] (biotin)
    HIV-1 p24 Antibody [8D7E11] (biotin)

    CATALOG NUMBER: PM-7367-biotin