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BAFF Antibody [Buffy-2]
CATALOG NUMBER: XA-1012- Clonality: Monoclonal
- Tested Applications: Flow, IHC, WB
- Host Species: Rat
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rat
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IHC, WB
- APPLICATION NOTE: BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC). After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..
Properties
- PURIFICATION: Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.
- CLONALITY: Monoclonal
- ISOTYPE: IgM
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: BAFF antibody can be stored at 4°C, stable for one year.
Additional Info
- NCBI GENE ID NUMBER: 10673
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- 1: Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002).
- 2: Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004).
- 3: Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004).
- 4: BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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