- SPECIES: Cynomolgus, Rhesus macaque
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Val 35 - Asn 242
- FUSION TAG: Fc Tag
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: This recombinant protein can be used for E, WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 50.5 kDa
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag with a linear range of 1-8 ng/mL.
- PURITY: >95% as determined by reduced SDS-PAGE.
Less than 1.0 EU per ug by the LAL method.
- PHYSICAL STATE: Lyophilized
- BUFFER: Tris with Glycine, Arginine and NaCl, pH7.5
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: B7-1
- ADDITIONAL NAMES: CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
- Protein Accession Number: G7NXN7
- NCBI GENE ID NUMBER: 102140078
- B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response.B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-kappaB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
- 1: Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
- 2: Stamper CC., et al., 2001, Nature (England) 410 (6828): 608–11.
- 3: Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
- 4: Khan N., et al., 2007, Int. Immunol. 19:477-486.
- FOR RESEARCH USE ONLY
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CATALOG NUMBER: 96-791
- Size: 0.1 mg
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