- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (Human beta-Nerve Growth Factor).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Neut, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hβ-NGF (1 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody is required.
To detect hβ-NGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hβ-NGF.
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
- PURIFICATION: Anti-hβ-NGF specific antibody was purified by affinity chromatography employing immobilized hβ-NGF matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: B-NGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: NGF
- ADDITIONAL NAMES: NGFB, HSAN5, Beta-NGF, NGFB, Beta-nerve growth factorBeta-NGF
- Protein Accession Number: P01138
- PROTEIN GI NUMBER: 90110037
- NCBI GENE ID NUMBER: 4803
- USER NOTE: Centrifuge vial prior to opening.
- FOR RESEARCH USE ONLY
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- Disclaimer: This product is for research use only.
CATALOG NUMBER: XP-5110
- Size: 0.1 mg
- List Price: $350.00
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