- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (85%)
- IMMUNOGEN: AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2.
The immunogen is located within amino acids 780 - 830 of AXIN2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
- SPECIFICITY: AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
- POSITIVE CONTROL 1: Cat. No. 1402 - Mouse Lung Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: 85, 93 kDa
- PURIFICATION: AXIN2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: AXIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: AXIN2
- ADDITIONAL NAMES: AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like protein, Axil
- Protein Accession Number: AAF22799
- PROTEIN GI NUMBER: 195927059
- NCBI GENE ID NUMBER: 8313
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
- 1: Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. Cancer Res.2007; 13:4042-5.
- 2: Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9.
- 3: Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and chromosome instability in cancer. Cell Cycle2006; 5:2077-81.
- 4: Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 6163
- Size: 0.02 mg | 0.1 mg
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