Autophagy Protein Detection Set
CATALOG NUMBER: PSI-1813- Clonality: Polyclonal
- Tested Applications: IF, IHC, WB
- Species Reactivity: Human
- Size: 1 Set
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- SPECIES REACTIVITY: Human
- IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
- TESTED APPLICATIONS: IF, IHC, WB
- SET CONTENTS: APG7 Antibody (CT), Catalog No. 3615 (50 μg)
Beclin-1 Antibody (CT), Catalog No. 3611 (50 μg)
LAMP1 Antibody, Catalog No. 3629 (50 μg)
LAMP2 Antibody, Catalog No. 3627 (50 μg)
PIST Antibody (CT), Catalog No. 3631 (50 μg)
Properties
- PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.
Additional Info
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity. LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression. However, the loss of both proteins results in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.
For images please see PDF data sheet - 1: Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- 2: Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- 3: Komatsu M, Waguri S, Ueno T, et al. Impairment of starvation-induced and constitutively autophagy in Atg7-deficient mice. J. Cell. Biol. 2005; 169:425-34.
- 4: Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
Set Contents
- Catalog Number 3615: APG7 Antibody
- Catalog Number 3611: Beclin-1 Antibody
- Catalog Number 3629: LAMP-1 Antibody
- Catalog Number 3627: LAMP-2 Antibody
- Catalog Number 3631: PIST Antibody
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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