ATP5J Antibody
CATALOG NUMBER: 19-147- Clonality: Polyclonal
- Tested Applications: IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 50 uL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, WB
- APPLICATION NOTE: WB: 1:200 - 1:2000
IF: 1:50 - 1:200 - POSITIVE CONTROL 1: SW480
- POSITIVE CONTROL 2: THP-1
- POSITIVE CONTROL 3: NCI-H460
- POSITIVE CONTROL 4: 22Rv1
- POSITIVE CONTROL 5: Mouse kidney
- POSITIVE CONTROL 6: Mouse heart
- PREDICTED MOLECULAR WEIGHT: Observed: 13kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: ATP5J
- ADDITIONAL NAMES: ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM
- NCBI GENE ID NUMBER: 522
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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