ATP5B Antibody
CATALOG NUMBER: 61-625- Clonality: Polyclonal
- Tested Applications: Flow, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
- IMMUNOGEN: This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC-P, WB
- APPLICATION NOTE: For IHC-P starting dilution is: 1:25
For IF starting dilution is: 1:25
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 57 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ATP5B
- ADDITIONAL NAMES: ATP synthase subunit beta, mitochondrial, ATP5B, ATPMB, ATPSB
- Protein Accession Number: P06576
- PROTEIN GI NUMBER: 114549
- NCBI GENE ID NUMBER: 506
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.
- 1: Neckelmann,N., et.al., Genomics(4), 829-843 (1989) Ohta,S., et.al., J. Biol. Chem. 263 (23), 11257-11262 (1988) Wallace,D.C., et.al., Curr. Genet. 12 (2), 81-90 (1987)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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