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ATP2C2 Antibody
CATALOG NUMBER: 6289- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: ATP2C2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP2C2.
The immunogen is located within the first 50 amino acids of ATP2C2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - SPECIFICITY: At least three isoforms of ATP2C2 are known to exist. ATP2C2 antibody will not cross-react with ATP2C1
- POSITIVE CONTROL 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-201 - 3T3/BALB Cell Slide
Properties
- PURIFICATION: ATP2C2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ATP2C2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ATP2C2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ATP2C2
- ADDITIONAL NAMES: ATP2C2 Antibody: SPCA2, KIAA0703, SPCA2, Calcium-transporting ATPase type 2C member 2, ATPase 2C2
- Protein Accession Number: NP_055676
- PROTEIN GI NUMBER: 118498343
- NCBI GENE ID NUMBER: 9914
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ATP2C2 Antibody: ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.
- 1: Xiang M, Mohamalawari D, and Rao R. A novel isoform of the secretory pathway Ca2+,Mn(2+)-ATPase, hSPCA2, has unusual properties and is expressed in the brain. J. Biol. Chem. 2005; 280:11608-14.
- 2: Hu Z, Bonifas JM, Beech J, et al. Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat. Genet. 2000; 24:61-5
- 3: Dode L, Andersen JP, Vanoevelen J, et al. Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses. J. Biol. Chem. 281:3182-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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