- Trial Size
ATG5 Antibody
CATALOG NUMBER: 4441- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (94%)
- IMMUNOGEN: ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.
The immunogen is located within the first 50 amino acids of ATG5. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
- POSITIVE CONTROL 1: Cat. No. 1466 - Rat Spleen Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Properties
- PURIFICATION: ATG5 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ATG5
- ADDITIONAL NAMES: ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
- Protein Accession Number: EAW48415
- PROTEIN GI NUMBER: 119568800
- NCBI GENE ID NUMBER: 9474
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
- 1: Gozuacik and Kimchi. Oncogene 2004; 23:2891-906.
- 2: Kisen et al. Carcinogenesis 1993; 14:2501-5.
- 3: Kamada et al. J. Cell. Biol. 2000; 150:1507-13.
- 4: Hanada et al. J. Biol. Chem.2007; 282:37298-302.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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