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ATF6 Antibody [AF6229]
CATALOG NUMBER: 33-034- Clonality: Monoclonal
- Tested Applications: IHC, WB
- Host Species: Mouse
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 100 ug
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- APPLICATION NOTE: Western blot: 2-4 ug/ml (2)
IHC (Paraffin): 5 ug/ml (1)
ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.
Properties
- PURIFICATION: Protein G purified monoclonal antibody
- CLONALITY: Monoclonal
- ISOTYPE: IgG1, kappa
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.5 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at -20°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: ATF6
- ADDITIONAL NAMES: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
- NCBI GENE ID NUMBER: 22926
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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