- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This ASPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human ASPH.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
- PREDICTED MOLECULAR WEIGHT: 86 kDa
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: ASPH
- ADDITIONAL NAMES: Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, ASP beta-hydroxylase, Peptide-aspartate beta-dioxygenase, ASPH, BAH
- Protein Accession Number: Q12797
- PROTEIN GI NUMBER: 145559444
- NCBI GENE ID NUMBER: 444
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- This gene is thought to play an important role in calciumhomeostasis. The gene is expressed from two promoters and undergoesextensive alternative splicing. The encoded set of proteins sharevarying amounts of overlap near their N-termini but havesubstantial variations in their C-terminal domains resulting indistinct functional properties. The longest isoforms (a and f)include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domainthat hydroxylates aspartic acid or asparagine residues in theepidermal growth factor (EGF)-like domains of some proteins,including protein C, coagulation factors VII, IX, and X, and thecomplement factors C1R and C1S. Other isoforms differ primarily inthe C-terminal sequence and lack the hydroxylase domain, and somehave been localized to the endoplasmic and sarcoplasmic reticulum.Some of these isoforms are found in complexes with calsequestrin,triadin, and the ryanodine receptor, and have been shown toregulate calcium release from the sarcoplasmic reticulum. Someisoforms have been implicated in metastasis.
- 1: Xue, T., et al. Hybridoma (Larchmt) 28(4):251-257(2009)
- 2: Luu, M., et al. Hum. Pathol. 40(5):639-644(2009)
- 3: Finotti, A., et al. BMC Mol. Biol. 9, 110 (2008) :
- 4: Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 56-642
- Size: 400 ul
- List Price: $430.00
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