Arrestin Antibody
CATALOG NUMBER: 45-295- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat
- IMMUNOGEN: The immunogen for this antibody is: C-HDHIPLPRPQS
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Spleen, Mouse Brain lysates and in preliminary testing of HEK293 cell lysate, and approx 45kDa in Mouse Spleen lysates and in preliminary testing of A549 cell lysate (calculated MW of 48kDa according to Human NP_001244257.1, 46.1kDa according to Human NP_004304.1; 46.3kDa according to Mouse NP_001258289.1 and 47.4kDa according to Mouse NP_001258287.1 ) Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
- SPECIFICITY: This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
- POSITIVE CONTROL 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: ARRB2
- ADDITIONAL NAMES: ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3
- Protein Accession Number: NP_004304.1,NP_945355.1,NP_001244257.1,NP_001244259.1,NP_001244260.1
- PROTEIN GI NUMBER: 4757780
- NCBI GENE ID NUMBER: 409
Background
- 1: Lefkowitz RJ, Shenoy SK. Transduction of receptor signals by beta-arrestins. Science. 2005 Apr 22;308(5721):512-7. Review.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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