- Trial Size
ARR3 Antibody
CATALOG NUMBER: 7643- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3.
The immunogen is located within the last 50 amino acids of ARR3. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - SPECIFICITY: ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.
- POSITIVE CONTROL 1: Cat. No. 1287 - EL4 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 43 kDa
Observed: 47 kDa
Properties
- PURIFICATION: ARR3 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ARR3 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ARR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: ARR3
- ADDITIONAL NAMES: ARR3 Antibody: ARRX, ARRX, CAR, Arrestin-C, Cone arrestin, C-arrestin
- Protein Accession Number: NP_004303
- PROTEIN GI NUMBER: 156071467
- NCBI GENE ID NUMBER: 407
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).
- 1: Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9.
- 2: Gurevich VV and Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther. 2006; 110:465-502.
- 3: Song X, Coffa S, Fu H, et al. How does arrestin assemble MAPKs into a signaling complex? J. Biol. Chem. 2009; 284:685-95.
- 4: Seo J, Tsakem EL, Breitman M, et al. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem. 2011; 286:27894-901.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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