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Home / E / ApoM Antibody [8F12C6B8]
ApoM Antibody [8F12C6B8]ApoM Antibody [8F12C6B8]
ApoM Antibody [8F12C6B8]
ApoM Antibody [8F12C6B8]

ApoM Antibody [8F12C6B8]

CATALOG NUMBER: 32-112
  • Clonality: Monoclonal
  • Tested Applications: E, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.1 mL

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3).
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, WB
  • APPLICATION NOTE: Western Blot:1:500 - 1:2,000
    IF:1:200 - 1:1,000
    ELISA:Propose dilution 1:10,000.
    Determining optimal working dilutions by titration test.

Properties

  • PURIFICATION: Antibody is purified by protein A affinity chromatography.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1 & IgG2b
  • BUFFER: Purified antibody in PBS containing 0.03% sodium azide.
  • CONCENTRATION: batch dependent
  • STORAGE CONDITIONS: ApoM monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: APOM
  • ADDITIONAL NAMES: G3A, NG20, HSPC336, Protein G3a, Apo-M, G3a, apo-M
  • Protein Accession Number: O95445
  • PROTEIN GI NUMBER: 17370872
  • NCBI GENE ID NUMBER: 55937
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21 kDa),is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.
  • 1: Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286.
  • 2: Duan J, Dahlback B, Villoutreix BO.FEBS Lett. 2001 Jun 15;499(1-2):127-32.
  • 3: Xu,N., Nilsson-Ehle,P. & Ahren,B. 2004. J. Nutr.Biochem. 15 (10):579-582.
  • 4: Zhang,X.Y. ,et al.2004. Acta Histochem. 106 (2):123-128

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 32-112

  • Size: 0.1 mL
  • List Price: $445.00
Datasheet
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