- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: APH1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human APH1a.
The immunogen is located within amino acids 40 - 90 of APH1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1283 - RAW 264.7 Cell Lysate
- PURIFICATION: APH1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: APH1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: APH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: APH1A
- ADDITIONAL NAMES: APH1 Antibody: APH-1, APH-1A, CGI-78, 6530402N02Rik, PSF, UNQ579/PRO1141, Gamma-secretase subunit APH-1A, Aph-1alpha, APH-1a
- Protein Accession Number: AAH08732
- PROTEIN GI NUMBER: 14250557
- NCBI GENE ID NUMBER: 51107
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- APH1 Antibody: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
- 1: Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA2002; 99:775-9.
- 2: Periz G and Fortini ME. Functional reconstitution of γsecretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
- 3: Selkoe DJ. The cell biology of βamyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.
- 4: Ma G, Li T, Price DL, et al. APH-1a is the principal mammalian aph-1 isoform present in g-secretase complexes during embryonic development. Neuro. Dis. 2005; 25:192-8.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 4001
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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