- Trial Size
APC7 Antibody
CATALOG NUMBER: 5731- Clonality: Polyclonal
- Tested Applications: ELISA, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: APC7 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human APC7.
The immunogen is located within amino acids 520 - 570 of APC7. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, WB
- APPLICATION NOTE: APC7 antibody can be used for detection of APC7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 66 kDa
Observed: 68 kDa
Properties
- PURIFICATION: APC7 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: APC7 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: APC7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ANAPC7
- ADDITIONAL NAMES: APC7 Antibody: APC7, APC7, Anaphase-promoting complex subunit 7, Cyclosome subunit 7
- Protein Accession Number: NP_057322
- PROTEIN GI NUMBER: 212549736
- NCBI GENE ID NUMBER: 51434
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- APC7 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators.
- 1: JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
- 2: Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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