- Trial Size
ANKLE2 Antibody
CATALOG NUMBER: 6609- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Chicken
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Chicken
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: ANKLE2 antibody was raised against a 19 amino acid synthetic peptide near the center of human ANKLE2.
The immunogen is located within amino acids 280 - 330 of ANKLE2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ANKLE2 antibody can be used for detection of ANKLE2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. - SPECIFICITY: Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE1.
- POSITIVE CONTROL 1: Cat. No. 1313 - Human Testis Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 121, 139, 145 kDa
Observed: 121 kDa
Properties
- PURIFICATION: ANKLE2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgY
- PHYSICAL STATE: Liquid
- BUFFER: ANKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ANKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ANKLE2
- ADDITIONAL NAMES: ANKLE2 Antibody: Lem4, LEMD7, KIAA0692, LEM4, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4
- Protein Accession Number: AAH43157
- PROTEIN GI NUMBER: 148664230
- NCBI GENE ID NUMBER: 23141
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ANKLE2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE2 (Ankyrin repeat and LEM domain containing 2) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains three ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. The function of this protein is still under investigation.
- 1: Lee KK and Wilson KL. All in the family: evidence for four new LEM-domain proteins Lem2 (NET-25), Lem3, Lem4 and Lem5 in the human genome. Symp. Soc. Exp. Biol. 2004; 56:329-39
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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