Specifications
- SPECIES: Human
- SOURCE SPECIES: High-5 Insect cells
- RECOMBINANT PROTEIN SEQUENCE: QKLSKHKTPA QPQLKAANCC EEVKELKAQV ANLSSLLSEL NKKQERDWVS VVMQVMELES NSKRMESRLT DAESKYSEMN NQIDIMQLQA AQTVTQTSAD AIYDCSSLYQ KNYRISGVYK LPPDDFLGSP ELEVFCDMET SGGGWTIIQR RKSGLVSFYR DWKQYKQGFG SIRGDFWLGN EHIHRLSRQP TRLRVEMEDW EGNLRYAEYS HFVLGNELNS YRLFLGNYTG NVGNDALQYH NNTAFSTKDK DNDNCLDKCA QLRKGGYWYN CCTDSNLNGV YYRLGEHNKH LDGITWYGWH GSTYSLKRVE MKIRPEDFKP HHHHHHHH
- BIOLOGICAL ACTIVITY: Measured by its binding ability to recombinant αvβ3 integrin in a functional ELISA.
Properties
- PURITY: ≥ 98% by SDS-PAGE gel and HPLC analyses.
- PHYSICAL STATE: Lyophilized
- STORAGE CONDITIONS: The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
- NCBI OFFICIAL SYMBOL: ANGPTL7
- ADDITIONAL NAMES: Angiopoietin-related protein 7, Angiopoietin-like protein 7, Cornea-derived transcript 6 protein, CDT6
- NCBI GENE ID NUMBER: 10218
Background
- Angiopoietin-like 7 (ANGPTL-7), or Cornea-Derived Transcript 6 (CDT6), is a member of the angiopoietin family of structurally related proteins, characterized by a coiled N-terminal domain and a C-terminal fibrinogen like domain. While ANGPTL-7 shares the structural features of the angiopoietin family, it plays a critical role in blocking the vascular endothelial Tie2 receptor to which other family members bind. Through the blocking of the Tie2 receptor, ANGPTL-7 does not act as a “true” angiopoietin, but rather as a morphogen that contributes to the avascularity and transparency of the cornea during both embryo and adult development. Human ANGPTL-7 is expressed at high levels in the avascular corneal stromal layer, a site of pathological angiogenesis normally devoid of blood vessels, suggesting that the protein acts as a negative regulator of angiogenesis in a manner similar to that of angiopoietin-1 and angiopoietin-2. In mouse xenograft models, ANGPTL-7overexpression has been shown to lead to increased extracellular matrix components typical of a mature corneal stromal layer, as well as the reduction of tumor growth and aberrant blood vessel formation. Overexpression in human melanoma models shows a contradictory, up-regulation of endostatin, an endogenous angiostatic factor, in comparison to the down-regulation observed in mouse models. Recombinant Human ANGPTL-7 is a glycoprotein that migrates by SDS-PAGE analysis at an apparent molecular weight of 40- 50 kDa under reduced conditions, and contains 328 amino acid residues including a C-terminal His-tag. The calculated molecular weight of Recombinant Human ANGPTL-7 is 38.2 kDa.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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