Close Hamburger Menu Close Hamburger Menu
Home / Detection Sets / Alzheimers Disease B-Amyloid Protein Detection Set
APP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyAPP AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE AntibodyBACE2 AntibodyBACE2 Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
APP Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE Antibody
BACE2 Antibody
BACE2 Antibody

Alzheimers Disease B-Amyloid Protein Detection Set

CATALOG NUMBER: PSI-1812
  • Clonality: Polyclonal
  • Tested Applications: IHC, WB
  • Species Reactivity: Human
  • Size: 1 Set

Specifications

  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
  • TESTED APPLICATIONS: IHC, WB
  • SET CONTENTS: APP Antibody (CT), Catalog No. 2136 (50 μg)

    APP Antibody (Aβ-NT), Catalog No. 2133 (50 μg)

    BACE Antibody (CT), Catalog No. 2253 (50 μg)

    BACE2 Antibody (NT), Catalog No. 2247 (50 μg)
  • APPLICATION NOTE: These polyclonal antibodies can be used for detection of APP, BACE and BACE2 by immunoblot at 1 - 5 μgg/mL. APP and BACE antibodies can detect their respective proteins via immunohistochemistry at 2 - 10 μg/mL.
  • SPECIFICITY: Both APP antibodies will react with the C99 fragment of APP.

Properties

  • PURIFICATION: Antibodies are supplied as affinity chromatography purified IgG.
  • CLONALITY: Polyclonal
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS containing 0.02% sodium azide.
  • CONCENTRATION: Antibody 1 mg/mL
  • STORAGE CONDITIONS: Stable at 4°C for three months, store at -20°C for up to one year.

Additional Info

  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The βamyloid protein precursor (APP) is cleaved by one of two βsecretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for βsecretase to generate the 4 kDa amyloid-β peptide (Aβ), which is deposited in the Alzheimer’s disease patients’ brains. BACE was identified by several groups independently and designated β-site APP cleaving enzyme (BACE) . BACE is a transmembrane aspartic protease and co-localizes with APP. BACE2 also cleaves APP at β-site and at a different site within Aβ. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and Aβ may also contribute to the pathogenesis of Down syndrome.

    For images please see PDF data sheet
  • 1: Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41.
  • 2: Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999; 402:537-40.
  • 3: Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59-64
  • 4: Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177-184.

Set Contents

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: This product is for research use only.

CATALOG NUMBER: PSI-1812

  • Size: 1 Set
  • List Price: $535.00
Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services 

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products
HIV-1 p24 Antibody [10A2] (biotin)
HIV-1 p24 Antibody [10A2] (biotin)

CATALOG NUMBER: PM-7371-biotin

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [8D7E11] (biotin)
HIV-1 p24 Antibody [8D7E11] (biotin)

CATALOG NUMBER: PM-7367-biotin