- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: alpha-Tubulin antibody is human, mouse and rat reactive.
- POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 50 kDa
Observed: 54 kDa
- PURIFICATION: alpha-Tubulin antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: TUBA1A
- ADDITIONAL NAMES: Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
- Protein Accession Number: CAA25855
- PROTEIN GI NUMBER: 37492
- NCBI GENE ID NUMBER: 7846
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
- 1: McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
- 2: Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
- 3: Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 7597
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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