Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The immunogen for this antibody is: C-HYKTDVAVKDDPE
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2ug/ml.
- POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: ALOX15
- ADDITIONAL NAMES: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2
- Protein Accession Number: NP_001131.3
- PROTEIN GI NUMBER: 40316937
- NCBI GENE ID NUMBER: 246
Background
- 1: McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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