Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Akt2 (Phospho-Ser474) antibody was raised against a peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- APPLICATION NOTE: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
- SPECIFICITY: This antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
- PREDICTED MOLECULAR WEIGHT: 60 kDa
Properties
- PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Store antibody at -20°C for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: AKT2
- ADDITIONAL NAMES: PKBB, PRKBB, HIHGHH, PKBBETA, RAC-BETA, IKKA, TCF16, PKB beta, Protein kinase B, RAC-PK-beta
- Protein Accession Number: NP_001617.1
- PROTEIN GI NUMBER: 4502023
- NCBI GENE ID NUMBER: 208
Background
- General protein kinase capable of phosphorylating several known proteins.
- 1: Sun M, et al. (2001) Cancer Res; 61 (16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19 (19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272 (48): 30491-7.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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