Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (81%)
- IMMUNOGEN: Akt1 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human Akt1.
The immunogen is located within amino acids 20 - 70 of Akt1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, IP, WB
- APPLICATION NOTE: Akt1 antibody can be used for the detection of Akt1 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1304 - Human Liver Tissue Lysate
Properties
- PURIFICATION: Akt1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Akt1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Akt1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: AKT1
- ADDITIONAL NAMES: Akt1 Antibody: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Protein kinase B
- Protein Accession Number: AAH00479
- PROTEIN GI NUMBER: 12653417
- NCBI GENE ID NUMBER: 207
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Akt1 Antibody: Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt, is part of the phosphatidyl 3'-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival. Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2. The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad. Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors.
- 1: Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science 1991; 254:274-7.
- 2: Song G, Ouyang G, and Bao S. The activation of Akt/PKB signaling pathway and cell survival. J. Cell. Mol. Med. 2005; 9:59-71.
- 3: Alessi DR, Adnjelkovic M, Caudwell B, et al. Mechanism of protein kinase B by insulin and IGF-1. EMBO J. 1996; 15:6541-51.
- 4: Nave BT, Ouwens M, Withers DJ, et al. Mammalian target of rapamycin is a direct target for protein kinase B: Identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem. J. 1999; 344:427-31.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
SARS-CoV-2 Omicron BA.1 Variant Pseudovirus
CATALOG NUMBER: 95-201
SARS-CoV-2 WT Pseudovirus
CATALOG NUMBER: 95-200
SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron)
CATALOG NUMBER: 9805