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AIFM2 (IN) Antibody
CATALOG NUMBER: 9655- Clonality: Polyclonal
- Tested Applications: E, IF, IHC, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Gorilla (100%); chimpanzee (100%), cow (100%), Horse (93%)
- IMMUNOGEN: Anti-AIFM2 antibody ( 9655) was raised against a peptide corresponding to 14 amino acids near the center of human AIFM2. The immunogen is located within 130-180 amino acids of AIFM2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC, WB
- APPLICATION NOTE: WB: 2 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1201 – HeLa Lysate
- POSITIVE CONTROL 2: Cat. No. 17-001 – HeLa Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 41kD
Observed: 40 kD
Advanced Validation
- ISOFORMS: Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD). Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD). Rat AIFM2 has one isoform (373aa, 40.7kD). 9653 can detect AIFM2 in human, mouse and rat.
Properties
- PURIFICATION: AIFM2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: AIMF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: AIMF2
- ADDITIONAL NAMES: AIMF2
- Protein Accession Number: Q9BRQ8
- PROTEIN GI NUMBER: 74752283
- NCBI GENE ID NUMBER: 84883
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro)viral) cytosolic DNA, thus contributing to the onset of apoptosis
- 1: Dai et al. Biochem Biophys Res Commun. 2020; 523(4):966-971
- 2: Bersuker et al. Nature 2019; 575(7784):688-692
- 3: Doll et al. Nature 2019; 575(7784):693-698
- 4: Chen et al. J Mol Neurosci 2019; 68(2): 304-310
- 5: Gong et al. J Biol Chem 2007; 282(41):30331-30340
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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