AIF1 Antibody
CATALOG NUMBER: 45-220- Clonality: Polyclonal
- Tested Applications: ELISA, Flow, IF, IHC, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog
- IMMUNOGEN: The immunogen for this antibody is: C-NKQFLDDPKYSSDED
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, IF, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.
- SPECIFICITY: This antibody is expected to recognise isoform 3 (NP_001614.3) only.
- POSITIVE CONTROL 1: Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: AIF1
- ADDITIONAL NAMES: AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
- Protein Accession Number: NP_001614.3
- PROTEIN GI NUMBER: 14574568
- NCBI GENE ID NUMBER: 199
Background
- 1: Yang ZF, Ho DW, Lau CK, Lam CT, Lum CT, Poon RT, Fan ST. Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages. Int Immunol. 2005 Nov;17(11):1391-7. Epub 2005 Sep 12.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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