- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Anti-AIF antibody (2267) was raised against a peptide corresponding to 15 amino acids near the center of human AIF.
The immunogen is located within amino acids 500-550 of AIF.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P, WB
- APPLICATION NOTE: WB: 1-2 μg/mL; IHC-P: 10 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
- SPECIFICITY: At least five isoforms of AIF are known to exist; this antibody will detect all isoforms except isoform 5.
- POSITIVE CONTROL 1: Cat. No. 1204 - K562 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1461 - Rat Heart Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 1401 - Mouse Heart Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 67 kDa
Observed: 68 kDa
Independent Antibody Validation in Cell lines (Figure 2) shows similar AIF expression profile in human and mouse cell lines detected by three independent anti-AIF antibodies that recognize different epitopes, 2267 against central domain, 2239 against N-terminus domain and 2301 against C-terminus domain. AIF proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies.
KD Validation (Figure 6,7): Anti-AIF antibody specificity was further verified by AIF specific knockdown. AIF signal in H1299 cells and AIF stable cells transfected with AIF siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.
Localization Validation (Figure 10): AIF detected by anti-AIF antibodies is colocalized with Mitotracker (mitochondrion marker). AIF is localized in mitochondria.
Induced Expression Validation (Figure 11): AIF expression detected by anit-AIF antibodies was up-regulated by Magnolol treatment.
- ISOFORMS: Human AIF has 6 isoforms, including isoform 1 (613aa, 67kD), isoform 2 (326aa, 36kD), isoform 3 (609aa, 66kD), isoform 4 (324aa, 35kD), isoform 5 (261aa, 28kD), and isoform 6 (237aa, 26kD). Mouse AIF has one isoform (612aa, 67kD) and Rat AIF also has one isoform (612aa, 67kD). 2267 can detect 4 human isoforms (1,2,3,5) and can also detect mouse and rat AIF.
- PURIFICATION: AIF Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: AIF Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: AIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: AIFM1
- ADDITIONAL NAMES: AIF Antibody: Apoptosis-inducing factor 1, Programmed cell death protein 8, AIF, PDCD8
- Protein Accession Number: O95381
- PROTEIN GI NUMBER: 50400606
- NCBI GENE ID NUMBER: 9131
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
- 1: Zamzami et al. Nature 1999; 401:127-8.
- 2: Susin et al. Nature 1999; 397:441-6
- 3: Daugas et al. FASEB J. 2000; 14:729-39
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- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 2267
- Size: 0.02 mg | 0.1 mg
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