Specifications
- SPECIES: Human
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Ser 21 - Ser 426
- FUSION TAG: His Tag
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: This recombinant protein can be used for E, WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 13.0 kDa (mature) and 32.0 kDa (pro)
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human Activin A, His Tag at 10 ug/mL (100 uL/well) can bind Human ACVR2A, Fc Tag with a linear range of 0.625-20 ug/mL.
Properties
- PURITY: >90% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method. - PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Activin A
- ADDITIONAL NAMES: Activin A,INHBA
- Protein Accession Number: AAH07858.1
- NCBI GENE ID NUMBER: 3624
Background
- Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion.Activins are nonglycosylated homodimers or heterodimers of various beta subunits (betaA, betaB, betaC, and betaE in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two betaA chains. The betaA subunit can also heterodimerize with a betaB or betaC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human betaA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat betaA.Activin is produced in the gonads, pituitary gland, placenta, and other organs. The bioactivity of Activin A is regulated by a variety of mechanisms. In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization; Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation; Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and especially kidney. Activin A increased the expression level of type-I collagen suggesting that activin A acts as a potent activator of fibroblasts; Lack of activin during development results in neural developmental defects.
- 1: Chen YG., et al., 2006, Exp. Biol. Med. (Maywood) 231 (5): 534–44.
- 2: Sulyok S, et al., 2004, Mol. Cell. Endocrinol. 225 (1–2): 127–32.
- 3: van Zonneveld P, et al., 2003, Hum Reprod 18 (3): 495–501.
- 4: Harrison, C.A. et al., 2005, Trends Endocrinol. Metab. 16:73.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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