Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
- IMMUNOGEN: Rabbit polyclonal ACSL1 antibody was raised against an 18 amino acid peptide near the center of human ACSL1.
The immunogen is located within amino acids 240 - 290 of ACSL1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: ACSL1 antibody can be used for detection of ACSL1 by Western blot at 0.25 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least three isoforms of ACSL1 are known to exist; this antibody will detect all three isoforms.
- POSITIVE CONTROL 1: Cat. No. 1304 – Human Liver Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-101 - Human Lung Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 41, 58, 77 kDa
Observed: 45, 47 kDa
Properties
- PURIFICATION: ACSL1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ACSL1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ACSL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ACSL1
- ADDITIONAL NAMES: ACSL1 Antibody: ACS1, LACS, FACL1, FACL2, LACS1, LACS2, Long-chain-fatty-acid--CoA ligase 1, Acyl-CoA synthetase 1, ACS1
- Protein Accession Number: NP_001986
- PROTEIN GI NUMBER: 40807491
- NCBI GENE ID NUMBER: 2180
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ACSL1 Antibody: Long-chain acyl coenzyme A synthetase 1 (ACSL1) catalyzes the synthesis of acyl-CoA from long-chain fatty acids in an ATP-dependent manner. ACSL1 is a member of a family of long-chain acyl-CoA synthetases which differ in substrate preference, tissue expression, and subcellular localization. In mouse, ACSL1 is the major acyl-CoA enzyme in the heart, providing 60-90% of heart ATP. Loss of ACSL1 either globally or in heart ventricles resulted in impaired fatty acid oxidation, activation of the mammalian target of rapamycin (mTOR), and cardiac hypertrophy.
- 1: Black PN and DiRusso CC. Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification. Microbiol. Mol. Biol. Rev. 2003; 67:454-72.
- 2: Coleman RA, Lewin TM, Van Horn CG, et al. Do acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways? J. Nutr. 2002; 132:2123-6.
- 3: Clark H, Carling D, and Saggerson D. Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids. Eur. J. Biochem. 2004; 271:2215-24
- 4: Ellis JM, Mentock SM, DePetrillo MA, et al. Mouse cardiac acyl Coenzyme A synthetase 1 deficiency impairs fatty acid oxidation and induces cardiac hypertrophy. Mol. Cell. Biol. 2011; 31:1252-62.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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