HIGH PERFORMANCE ANTIBODIES … AND MORE

Recombinant Monoclonal

Recombinant Monoclonal Antibodies

Recombinant monoclonal antibodies are highly specific, high-affinity antibodies that are able to be created in vitro. Recombinant antibodies have been explored as a treatment for cancer, HIV, HSV as well as a variety of other human disorders. These antibodies are also smaller than traditional monoclonal antibodies which have been shown to allow them to better distribute in patients treated with recombinant antibodies.

Key Advantages:

  • Reduced production time
  • Can be created in vitro
  • High Specificity

For more information on monoclonal antibodies, check out our blog: Antibody Basics: Monoclonal, Polyclonal, and Recombinant Antibodies!

    Primary Antibodies

  • ACE2 Antibody, Monoclonal

    Catalog Number: 24-033

    Clonality: Monoclonal

    Tested Applications: IHC, WB

    Species Reactivity: Virus

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    3 Image(s)

    • <strong>Figure 1 Western Blot Validation in Mouse and Rat Tissue Lysates</strong><br> Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, 24-033, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
    • <strong>Figure 2 Western Blot Validation in HepG2 Cells</strong><br> Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, 24-033, at 1:500 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
    • <strong>Figure 3 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue</strong><br> Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, 24-033, at 1:100 dilution (40x lens).

  • Recombinant CD20 Antibody [RMC20-1]

    Catalog Number: 34-059

    Clonality: Monoclonal

    Tested Applications: IHC

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ug

    1 Image(s)

    • IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. HIER: steam section in pH6 citrate buffer for 20 min.

  • Recombinant CD31 Antibody [RMCD31-1]

    Catalog Number: 34-035

    Clonality: Monoclonal

    Tested Applications: IF, IHC

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ug

    1 Image(s)

    • IHC staining of FFPE human angiocarcinoma with CD31 antibody.

  • Recombinant MART-1 / Melan-A Antibody [RMMRT1-1]

    Catalog Number: 34-033

    Clonality: Monoclonal

    Tested Applications: IHC

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ug

    2 Image(s)

    • Formalin-fixed, paraffin-embedded human melanoma stained with MART-1 antibody (RMMRT1-1).
    • Formalin-fixed, paraffin-embedded human melanoma stained with MART-1 antibody (RMMRT1-1).

  • Recombinant NKX2.2 Antibody [RMNK2-1]

    Catalog Number: 34-046

    Clonality: Monoclonal

    Tested Applications: IF, IHC

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ug

    1 Image(s)

    • IHC testing of FFPE human pancreas with NKX2.2 antibody.

  • SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [ARC2372]

    Catalog Number: 24-031

    Clonality: Monoclonal

    Tested Applications: ELISA, Flow, IF, IHC, IP, WB

    Species Reactivity: Virus

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • <strong>Figure 1 Western Blot Validation</strong><br> Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody, 24-031, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.

  • SARS-CoV-2 (COVID-19) Spike Antibody [ARC2373]

    Catalog Number: 24-032

    Clonality: Monoclonal

    Tested Applications: ELISA, Flow, IF, IHC, IP

    Species Reactivity: Virus

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • <strong>Figure 1 Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in HeLa cells</strong><br> Immunofluorescence analysis of HeLa cells, untreated (right) and SARS-CoV-2 (COVID-19) spike plasmid transfected (left) using SARS-CoV-2 (COVID-19) spike antibody, 24-032, at 1:100 dilution. Blue: DAPI for nuclear staining.

  • SARS-CoV-2 Nucleocapsid Antibody

    Catalog Number: 10-065

    Clonality: Monoclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Virus

    Conjugate: Unconjugated

    Size: 0.1 mg

    3 Image(s)

    • Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15min), blocked with 4 % BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour), Image Courtesy of Dr. Ana Fernandex - Sesma Laboratory, ISMMS.
    • Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.
    • Exp1293 cells were transfected with expression plasmid coding for SARs2 NP and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supermatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.

  • SARS-CoV-2 Spike NTD Antibody [2146]

    Catalog Number: 10-055

    Clonality: Monoclonal

    Tested Applications: ELISA, IHC

    Species Reactivity: Virus

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8˚C. Detection: Anti-SARS-CoV-2 NTD (Clone 2146) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37˚C. Substrate: TMB (Leinco T118), 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.

  • SARS-CoV-2 Spike NTD Antibody [2146] (Biotin)

    Catalog Number: 10-056

    Clonality: Monoclonal

    Tested Applications: ELISA, IHC

    Species Reactivity: Virus

    Conjugate: Biotin

    Size: 0.05 mg

    1 Image(s)

    • Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8˚C. Detection: Anti-SARS-CoV-2 NTD (Clone 2146) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37˚C. Substrate: TMB (Leinco T118), 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.
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