HIGH PERFORMANCE ANTIBODIES … AND MORE

Phospho-specific

Post-translational phosphorylation of proteins plays an important role in many regulatory mechanisms such as cell cycle, proliferation, metabolism, and growth and development. Disorders like cardiovascular disease, cancer, and diabetes have errors in phosphorylation associated with them. ProSci offers a large selection of phospho-specific antibodies to help researchers uncover new information about the role of post-translational phosphorylation.

    Primary Antibodies

  • 14-3-3 (phospho Ser58) Antibody

    Catalog Number: 50-231

    Clonality: Polyclonal

    Tested Applications: IHC, WB

    Species Reactivity: Bovine, Chicken, Dog, Human, Mouse, Rat, Sheep, Xenopus, Zebrafish

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mL

    1 Image(s)

    • Western blot of rat brainstem lysate showing specific immunolabeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).

  • 14-3-3 zeta/ delta (phospho Thr232) Antibody

    Catalog Number: 80-004

    Clonality: Polyclonal

    Tested Applications: WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) Antibody. The lane on the right is treated with the antigen-specific peptide.

  • 4E BP1 (phospho Thr45) Antibody

    Catalog Number: 79-205

    Clonality: Polyclonal

    Tested Applications: IHC, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).

  • 4E-BP1 (phospho Thr70) Antibody

    Catalog Number: 79-891

    Clonality: Polyclonal

    Tested Applications: IHC

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • 5 Lipoxygenase (phospho Ser523) Antibody

    Catalog Number: 50-244

    Clonality: Polyclonal

    Tested Applications: WB

    Species Reactivity: Human, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mL

    1 Image(s)

    • Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with lamda-Ptase.

  • a catenin (phospho Ser641) Antibody

    Catalog Number: 79-503

    Clonality: Polyclonal

    Tested Applications: WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Western blot analysis of lysed extracts from HeLa cells untreated or treated with starvation using α-catenin (Phospho-Ser641).

  • a Synuclein (phospho Ser129) Antibody

    Catalog Number: 79-461

    Clonality: Polyclonal

    Tested Applications: WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Western blot analysis of lysed extracts from mouse brain tissue using α-Synuclein (Phospho-Ser129).

  • a Synuclein (phospho Tyr125) Antibody

    Catalog Number: 79-483

    Clonality: Polyclonal

    Tested Applications: IF, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from C2C12 cells untreated or treated with Anisomycin using α-Synuclein (Phospho-Tyr125).
    • Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (Phospho-Tyr125).

  • a Synuclein (phospho Tyr136) Antibody

    Catalog Number: 79-491

    Clonality: Polyclonal

    Tested Applications: IF, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from mouse brain tissue using α-Synuclein (Phospho-Tyr136).
    • Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (Phospho-Tyr136).

  • Abl (phospho Tyr204) Antibody

    Catalog Number: 79-953

    Clonality: Polyclonal

    Tested Applications: WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • Western blot analysis of extracts from COS7 cells treated with Adriamycin using Abl (Phospho-Tyr204) Antibody. The lane on the right is treated with the antigen-specific peptide.
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