HIGH PERFORMANCE ANTIBODIES … AND MORE

Infectious Disease

    Primary Antibodies

  • CD81 Antibody [1.3.3.22]

    Catalog Number: 33-829

    Clonality: Monoclonal

    Tested Applications: Flow, Func, IF, IHC-P, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Mouse

    Conjugate: Unconjugated

    Size: 100 ug

    2 Image(s)

    • IHC testing of FFPE human lymph node with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
    • IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

  • CXCR4 Antibody

    Catalog Number: 1012

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    9 Image(s)

    • <strong>Figure 1 Western Blot Validation of CXCR4 in HeLa Cells </strong><br> Loading: 15 μg of lysates per lane. Antibodies: 1012 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile</strong><br> Loading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with CXCR4 siRNA Knockdown in HeLa Cells </strong><br> HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1012 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Animal Species Reactivity </strong><br> Loading: Lysates/proteins at 20 μg per lane. Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 5 Immunofluorescence Validation of CXCR4 </strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1012 at 4 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cell line.
    • <strong>Figure 6 Immunohistochemistry Validation of CXCR4 in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1012) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
    • <strong>Figure 7 KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010) </strong><br> Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4−/− astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).
    • <strong>Figure 8 Overexpression Validation of CXCR4 (Kozak et al., 2002) </strong><br> U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.
    • <strong>Figure 9 WB Validation of CXCR4 in Human Metastatic Melanoma (Scala et al., 2006) </strong><br> CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.
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