HIGH PERFORMANCE ANTIBODIES … AND MORE

Infectious Disease

    Primary Antibodies

  • ATR Antibody

    Catalog Number: 3119

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, IF, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • Western blot analysis of ATR in K562 cell lysates with ATR antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
    • Immunocytochemistry of ATR in K562 cells with ATR antibody at 2 μg/mL.
    • Immunofluorescence of ATR in K562 cells with ATR antibody at 10 μg/mL.

  • CXCR4 Antibody

    Catalog Number: 1009

    Clonality: Polyclonal

    Tested Applications: ELISA, Flow, ICC, IF, IHC-P, IP, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    12 Image(s)

    • <strong>Figure 1 Western Blot Validation of CXCR4 in HeLa Cells </strong><br> Loading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with CXCR4 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: 1009 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Animal Species Reactivity </strong><br> Loading: Lysates/proteins at 20 μg per lane. Antibodies: 1009 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 5 Recombinant Protein Test </strong><br> Loading: CXCR4 partial recombinant protein (Novus Biologicals, Cat# H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 6 Immunofluorescence Validation of CXCR4 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1009 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.
    • <strong>Figure 7 Flow Cytometry Validation of CXCR4 in HeLa Cells</strong><br> Overlay histogram showing HeLa cells stained with 1009 (red line, 1μg/1x106 cells). 1 h incubation at 4˚C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4˚C. <br> <br> Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions.
    • <strong>Figure 8 Immunohistochemistry Validation of CXCR4 in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1009) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
    • <strong>Figure 9 Immunocytochemistry Validation of CXCR4 in HeLa Cells </strong><br> Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody (1009) at 2 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
    • <strong>Figure 10 KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010) </strong><br> Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4−/− astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).
    • <strong>Figure 11 Overexpression Validation of CXCR4 (Kozak et al., 2002) </strong><br> U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.
    • <strong>Figure 12 WB Validation of CXCR4 in Human Metastatic Melanoma (Scala et al., 2006) </strong><br> CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.

  • DDX3 Antibody

    Catalog Number: 3759

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    2 Image(s)

    • Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 1 and (B) 2 μg/mL.
    • Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.

  • GPR15 Antibody

    Catalog Number: 2101

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    2 Image(s)

    • Western blot analysis of GPR15 in human spleen lysate with GPR15 antibody at (A) 0.5 and (B) 1 μg/mL.
    • Immunocytochemistry of GPR15 in A431 cells with GPR15 antibody at 5 μg/ml.
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