HIGH PERFORMANCE ANTIBODIES … AND MORE

Growth Factors

    Primary Antibodies

  • Nrac Antibody

    Catalog Number: 43-664

    Clonality: Polyclonal

    Tested Applications: ELISA

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    No Image

  • THNSL2 Antibody

    Catalog Number: 43-222

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • 43-222 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • TIM-1 Antibody

    Catalog Number: 3809

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    6 Image(s)

    • <strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with TIM-1 siRNA Knockdown in Hela Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3809 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Western Blot Validation in the Mouse Cell Line</strong><br> Loading: 15 μg of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Immunofluorescence Validation of TIM-1 in Human Uterus Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.
    • <strong>Figure 5 Immunohistochemistry Validation of TIM-1 in Human Uterus Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • TIM-1 Antibody

    Catalog Number: 3811

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • <strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with TIM-1 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

  • TIM-4 Antibody

    Catalog Number: 3815

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    6 Image(s)

    • Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
    • Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.
    • Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.
    • Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.
    • Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.<br><br>Green: TIM-4 Antibody (3815) <br> Blue: DAPI staining
    • Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 μg/ml.

  • TIM-4 Antibody

    Catalog Number: 3813

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    1 Image(s)

    • Western blot analysis of TIM-4 in Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 μg/mL.

  • VLCAD Antibody

    Catalog Number: 43-327

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • 43-327 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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