HIGH PERFORMANCE ANTIBODIES … AND MORE

Growth Factors

    Primary Antibodies

  • GFR alpha 1 Antibody

    Catalog Number: 1133

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    4 Image(s)

    • Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.
    • Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 μg/ml.
    • Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 μg/mL.<br><br>Red: GFR alpha 1 Antibody (1133) <br> Blue: DAPI staining
    • Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/mL.

  • GFR alpha 2 Antibody

    Catalog Number: 1135

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    2 Image(s)

    • Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.
    • Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.

  • GFR alpha 3 Antibody

    Catalog Number: 1137

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    4 Image(s)

    • Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 μg/mL.
    • Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 μg/mL.
    • Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml. <br><br>Green: GFR alpha 3 antibody (1137) <br> Red: Phylloidin staining<br> Blue: DAPI staining
    • Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml.

  • Lano Antibody

    Catalog Number: 3001

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • Western blot analysis of Lano expression in A431 cell lysate with Lano antibody at (A) 1 and (B) 2 μg/ml.
    • Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/mL.
    • Immunofluorescence of Lano in human colon tissue with Lano antibody at 20 μg/mL.

  • Neurturin Antibody

    Catalog Number: 1121

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    6 Image(s)

    • Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.
    • Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.
    • Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.<br><br>Green: Neuriturin Antibody (1121) <br> Red: Phylloidin staining<br> Blue: DAPI staining
    • Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.
    • Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 μg/mL.
    • Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 μg/mL.<br><br>Green: Neuriturin Antibody (1121) <br> Red: Phylloidin staining<br> Blue: DAPI staining

  • NGFR Antibody

    Catalog Number: 3593

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, IF, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • Western blot analysis of (A) 25 ng, (B) 50 ng, and (C) 100 ng of purified recombinant NGFR with NGFR antibody at 1 μg/mL.
    • Immunocytochemistry of NGFR in A20 cells with NGFR antibody at 10 μg/mL.
    • Immunofluorescence of NGFR in HepG2 cells with NGFR antibody at 20 μg/mL.<br><br>Green: NGFR Antibody (3593) <br> Blue: DAPI staining

  • TIM-1 Antibody

    Catalog Number: 3809

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    6 Image(s)

    • <strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with TIM-1 siRNA Knockdown in Hela Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3809 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Western Blot Validation in the Mouse Cell Line</strong><br> Loading: 15 μg of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 4 Immunofluorescence Validation of TIM-1 in Human Uterus Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.
    • <strong>Figure 5 Immunohistochemistry Validation of TIM-1 in Human Uterus Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • TIM-1 Antibody

    Catalog Number: 3811

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • <strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
    • <strong>Figure 3 Validation with TIM-1 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 μg of HeLa whole cell lysates per lane. Antibodies: 3811 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

  • TIM-4 Antibody

    Catalog Number: 3815

    Clonality: Polyclonal

    Tested Applications: ELISA, ICC, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    6 Image(s)

    • Western blot analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
    • Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 μg/mL.
    • Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 μg/mL.
    • Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 μg/ml.
    • Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 μg/ml.<br><br>Green: TIM-4 Antibody (3815) <br> Blue: DAPI staining
    • Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 μg/ml.

  • TIM-4 Antibody

    Catalog Number: 3813

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    1 Image(s)

    • Western blot analysis of TIM-4 in Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 μg/mL.
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