HIGH PERFORMANCE ANTIBODIES … AND MORE

Cancer

    Primary Antibodies

  • 14 3 3 zeta (Ab 58) Antibody

    Catalog Number: 79-354

    Clonality: Polyclonal

    Tested Applications: IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from HepG2 , HeLa and 293 cells using 14-3-3 zeta (Ab-58).
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Ab-58).

  • 14-3-3 sigma Antibody

    Catalog Number: 45-174

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    4 Image(s)

    • 45-174 (0.03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    • 45-174 (2ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
    • 45-174 (0.03ug/ml) staining of A431 (A) and U2OS (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
    • 45-174 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

  • 14-3-3 tau Antibody

    Catalog Number: 45-175

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • 45-175 (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

  • 27Kip1 Antibody

    Catalog Number: 62-207

    Clonality: Polyclonal

    Tested Applications: IF, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 400 ul

    2 Image(s)

    • Western Blot in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.
    • Confocal immunofluorescent analysis of 27Kip1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • 4E-T Antibody

    Catalog Number: 45-177

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • 45-177 staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • 45-177 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • 53BP1 Antibody

    Catalog Number: 19-839

    Clonality: Polyclonal

    Tested Applications: IF, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 50 ul

    6 Image(s)

    • Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (19-839) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
    • Immunohistochemistry of paraffin-embedded rat kidney using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human lung cancer using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded mouse heart using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunofluorescence analysis of U2OS cells using 53BP1 antibody (19-839). Blue: DAPI for nuclear staining.
    • Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (19-839). Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

  • A-RAF Antibody

    Catalog Number: 63-215

    Clonality: Polyclonal

    Tested Applications: IHC-P, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 400 ul

    2 Image(s)

    • Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35ug/lane)
    • Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

  • A2BP1 Antibody

    Catalog Number: 27-147

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human Brain at 0.2-1 ug/ml.

  • A4GNT Antibody

    Catalog Number: 26-196

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

  • A4GNT Antibody

    Catalog Number: 30-272

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
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Modifications
  1. Acetylated (1)
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Species Reactivity
  1. African green monkey (6)
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  38. Rabbit (26)
  39. Rat (3084)
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  42. Virus (4)
  43. Xenopus (7)
  44. Yeast (2)
  45. Zebrafish (83)
  46. Zika (3)
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Clonality
  1. Monoclonal (1097)
  2. Polyclonal (7646)

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