HIGH PERFORMANCE ANTIBODIES … AND MORE

Cancer

Cancer Research

Cancer is caused when changes in DNA lead to deregulation of cell growth in tissue. There are a variety of genes and their proteins that are associated with the creation and proliferation of cancer cells. Hundreds of thousands of people die from cancer each year in the United States alone. ProSci offers 1000s of antibodies to aid in the research of cancer and how it can be treated.

    Primary Antibodies

  • 14 3 3 zeta (Ab 58) Antibody

    Catalog Number: 79-354

    Clonality: Polyclonal

    Tested Applications: IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from HepG2 , HeLa and 293 cells using 14-3-3 zeta (Ab-58).
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Ab-58).

  • 14-3-3 sigma Antibody

    Catalog Number: 45-174

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    4 Image(s)

    • 45-174 (0.03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    • 45-174 (2ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
    • 45-174 (0.03ug/ml) staining of A431 (A) and U2OS (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
    • 45-174 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

  • 14-3-3 tau Antibody

    Catalog Number: 45-175

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    1 Image(s)

    • 45-175 (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

  • 27Kip1 Antibody

    Catalog Number: 62-207

    Clonality: Polyclonal

    Tested Applications: IF, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 400 ul

    2 Image(s)

    • Western Blot in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab.
    • Confocal immunofluorescent analysis of 27Kip1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • 4E-T Antibody

    Catalog Number: 45-177

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human

    Host Species: Goat

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • 45-177 staining (0.25ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • 45-177 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • 53BP1 Antibody

    Catalog Number: 19-839

    Clonality: Polyclonal

    Tested Applications: IF, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 50 ul

    6 Image(s)

    • Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (19-839) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
    • Immunohistochemistry of paraffin-embedded rat kidney using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human lung cancer using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded mouse heart using 53BP1 antibody (19-839) at dilution of 1:100 (40x lens).
    • Immunofluorescence analysis of U2OS cells using 53BP1 antibody (19-839). Blue: DAPI for nuclear staining.
    • Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (19-839). Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

  • A-RAF Antibody

    Catalog Number: 63-215

    Clonality: Polyclonal

    Tested Applications: IHC-P, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 400 ul

    2 Image(s)

    • Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35ug/lane)
    • Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

  • A2BP1 Antibody

    Catalog Number: 27-147

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human Brain at 0.2-1 ug/ml.

  • A4GNT Antibody

    Catalog Number: 26-196

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

  • A4GNT Antibody

    Catalog Number: 30-272

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
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