HIGH PERFORMANCE ANTIBODIES … AND MORE

Autophagy

    Primary Antibodies

  • ATG5 Antibody [1358CT289.125.123]

    Catalog Number: 64-229

    Clonality: Monoclonal

    Tested Applications: WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Western blot analysis of lysates from rat C6,Hela,mouse NIH/3T3,SH-SY5Y,U-87 MG cell line (from left to right), using ATG5 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.

  • CD107b Antibody [ABL-93]

    Catalog Number: 99-112

    Clonality: Monoclonal

    Tested Applications: Flow

    Species Reactivity: Mouse

    Host Species: Rat

    Conjugate: Unconjugated

    Size: 0.5 mg

    1 Image(s)

    • Osteoclasts from ashen mice were stained with AF594 conjugated phalloidin and Rat Anti-Mouse CD107b-UNLB (Cat. No. 99-112) followed by a secondary antibody and DAPI. Images from Shimada-Sugawara M, Sakai E, Okamoto K, Fukuda M, Izumi T, Yoshida N, et al. Rab27A regulates transport of cell surface receptors modulating multinucleation and lysosome-related organelles in osteoclasts. Sci Rep. 2015;5:9620. Figure 6(a)

  • LAMP2 Antibody [A1H9]

    Catalog Number: 13-868

    Clonality: Monoclonal

    Tested Applications: IHC, IP, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 50 ul

    7 Image(s)

    • Western blot analysis of extracts of JAR cells, using LAMP2 antibody (13-868).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
    • Immunohistochemistry of paraffin-embedded mouse pancreas using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human liver using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human kidney using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human pancreas using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded mouse kidney using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded mouse placenta using LAMP2 antibody (13-868) at dilution of 1:100 (40x lens).

  • MAP1LC3A Antibody [E2F7]

    Catalog Number: 14-019

    Clonality: Monoclonal

    Tested Applications: IF, IHC, IP, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 50 ul

    7 Image(s)

    • Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (14-019).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
    • Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded human liver using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded mouse liver using MAP1LC3A antibody (14-019) at dilution of 1:100 (40x lens).
    • Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody (14-019).
    • Immunofluorescence analysis of PC-12 cells using MAP1LC3A antibody (14-019).
    • Immunofluorescence analysis of HUVEC cells using MAP1LC3A antibody (14-019).

  • PRKAB1 Antibody [1F9G6]

    Catalog Number: 13-300

    Clonality: Monoclonal

    Tested Applications: ICC, IHC, IP, WB

    Species Reactivity: Human, Monkey, Mouse, Rat

    Host Species: Mouse

    Conjugate: Unconjugated

    Size: 50 ul

    1 Image(s)

    • Western blot analysis of extracts of various cell lines, using PRKAB1 antibody (13-300) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.

  • Ubiquitin Hydrolase 1 Antibody [MCA-BH7]

    Catalog Number: 50-263

    Clonality: Monoclonal

    Tested Applications: IHC, WB

    Species Reactivity: Bovine, Human, Mouse, Rat

    Host Species: Mouse

    Conjugate: Unconjugated

    Size: 0.1 mL

    1 Image(s)

    • Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. <br><br> <b>Below:</b> Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.
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