HIGH PERFORMANCE ANTIBODIES … AND MORE

Apoptosis

Apoptosis Research

Apoptosis is a process of programmed cell death that removes old and damaged cells to maintain homeostasis in multicellular organisms. The term “apoptosis” was adopted from the Greek word for the process of leaves falling from trees or petals falling from flowers (1) and was adopted to differentiate naturally occurring developmental cell death from the necrosis that results from acute tissue injury.

Apoptosis follows a scripted choreography of biochemical events that lead to characteristic morphological changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and complete mRNA decay, and subsequent cell death. The average human adult loses about 50 billion cells a day to apoptosis which is important in developmental regulation and maintaining a healthy body.

Errors in the regulation of apoptosis are associated with a variety of disorders such as cancers and the progression of HIV to AIDS. ProSci offers over 1500 apoptosis antibodies to help researchers gain new information about apoptosis and the role it plays in human diseases.

(1)  Kerr JF, Wyllie AH, Currie AR (August 1972). "Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics". Br J Cancer. 26 (4): 239–57

    Primary Antibodies

  • 14 3 3 zeta (Ab 58) Antibody

    Catalog Number: 79-354

    Clonality: Polyclonal

    Tested Applications: IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.1 mg

    2 Image(s)

    • Western blot analysis of lysed extracts from HepG2 , HeLa and 293 cells using 14-3-3 zeta (Ab-58).
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Ab-58).

  • AATF Antibody

    Catalog Number: 3039

    Clonality: Polyclonal

    Tested Applications: ELISA, IF, IHC-P, WB

    Species Reactivity: Human, Mouse

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 0.02 mg, 0.1 mg

    3 Image(s)

    • Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
    • Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.
    • Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.

  • AATF Antibody

    Catalog Number: 27-426

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    3 Image(s)

    • Antibody used in IHC on Human Intestine.
    • Antibody used in IHC on Human Liver cell lysates.
    • Antibody used in WB on Human kidney at 0.2-1 ug/ml.

  • AATF Antibody

    Catalog Number: 28-979

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human Daudi at 5.0 ug/ml.

  • AATF Antibody

    Catalog Number: 28-980

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Dog, Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human kidney at 0.25 ug/ml.

  • ABL1 Antibody

    Catalog Number: 13-126

    Clonality: Polyclonal

    Tested Applications: IF, IHC, WB

    Species Reactivity: Human, Mouse, Rat

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 50 ul

    4 Image(s)

    • Western blot analysis of extracts of HeLa cells, using ABL1 antibody (13-126) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
    • Immunohistochemistry of paraffin-embedded human prostate using ABL1 antibody (13-126) at dilution of 1:100 (40x lens).
    • Immunohistochemistry of paraffin-embedded rat kidney using ABL1 antibody (13-126) at dilution of 1:100 (40x lens).
    • Immunofluorescence analysis of A431 cells using ABL1 antibody (13-126) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

  • ACADM Antibody

    Catalog Number: 25-092

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

  • ACADM Antibody

    Catalog Number: 27-708

    Clonality: Polyclonal

    Tested Applications: ELISA, WB

    Species Reactivity: Dog, Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    1 Image(s)

    • Antibody used in WB on Human HepG2 at 5.0 ug/ml.

  • ACAT2 Antibody

    Catalog Number: 27-709

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    2 Image(s)

    • Antibody used in IHC on Human Liver.
    • Antibody used in WB on Human K562 at 1 ug/ml.

  • ACAT2 Antibody

    Catalog Number: 27-710

    Clonality: Polyclonal

    Tested Applications: ELISA, IHC, WB

    Species Reactivity: Human

    Host Species: Rabbit

    Conjugate: Unconjugated

    Size: 100 ul

    2 Image(s)

    • Antibody used in IHC on Human Stomach.
    • Antibody used in WB on Human K562 at 2.0 ug/ml.
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