PRICKLE1 Antibody Cat. No.: 6561

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1.

The immunogen is located within the last 50 amino acids of PRICKLE1.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
POSITIVE CONTROL:1) Cat. No. 1310 - Human Bladder Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 46, 91 kDa

Observed: 52, 90 kDa

psi-iconProperties
PURIFICATION:PRICKLE1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PRICKLE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PRICKLE1
ALTERNATE NAMES:PRICKLE1 Antibody: RILP, EPM1B, RILP, Prickle-like protein 1
ACCESSION NO.:NP_694571
PROTEIN GI NO.:222136684
GENE ID:144165
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.
REFERENCES:1) Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56
2) Shimojo M and Hersh LB. REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. Mol. Cell. Biol. 2003; 23:9025-31
3) Chan DW, Chan CY, Yam JW, et al. Prickle1 negatively regulates Wnt/β-catenin pathway by promoting Disheveled ubiquitination/degradation in liver cancer. Gastroenterology 2006; 131:1218-27.
4) Tao H, Manak JR, Sowers L, et al. Mutations in prickle orthologs cause seizures in flies, mice, and humans. Am. J. Hum. Genet. 2011; 88:138-49.

ANTIBODIES FOR RESEARCH USE ONLY.

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