Poly Antibody [10H] Cat. No.: 36-169

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Drosophila, Human, Mouse, Rat
IMMUNOGEN: Purified poly(ADP-ribose).
TESTED APPLICATIONS: Flow, ICC, IHC, WB
APPLICATIONS: Flow Cytometry: (see A. Kunzmann, et al.; Immun. Ageing 3, 8 (2006)). Immunocytochemistry: (5-20ug/ml). Immunohistochemistry (paraffin sections): Dilute in 5% non fat dried milk in PBS to a final concentration of 5-20ug/ml). Western Blot: Incubate 2.5ug/ml in PBS, 0.05% Tween20, 5% non fat dried milk. Optimal conditions must be determined individually for each application.
SPECIFICITY: Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases), including human, mouse, rat or Drosophila PARP enzymes.

psi-iconProperties
PURIFICATION:>95% (SDS-PAGE)
CLONALITY:Monoclonal
ISOTYPE:IgG3, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. Containing 50mM HEPES, pH 7.4, 100mM NaCl, 1% BSA and 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -80˚C.

psi-iconAdditional Info
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Processes such as transcription, repair and replication that require efficient DNA recognition are dependent on modulation of chromatin structure. Chromatin relaxation is a critical event that occurs during DNA repair and is associated with the negatively charged polymer of adenosine 5'-diphosphate (ADP)-ribose (PAR). PAR is synthesized from nicotinamide adenine dinucleotide (NAD+) by the poly(ADP-ribose) polymerase protein family (PARPs), of which PARP-1 (and to a lesser extent PARP-2) respond to DNA-strand breaks. PARP-1 is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself. The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). PAR regulates not only cell survival and cell-death programmes, but also an increasing number of other biological functions with which novel members of the PARP family have been associated. These include transcriptional regulation, cell division, intracellular trafficking, inflammation and energy metabolism.

ANTIBODIES FOR RESEARCH USE ONLY.

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