POLR2A Antibody Cat. No.: 13-946

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Pig, Rat
IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
TESTED APPLICATIONS: ChIP, IF, IHC, IP, WB
APPLICATIONS: WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

IP: ,1:20 - 1:50

ChIP: ,1:20 - 1:50
POSITIVE CONTROL:1) HeLa
 2) BT-474
 3) H460
 4) Jurkat
 5) Mouse testis
 6) Mouse brain
PREDICTED MOLECULAR WEIGHT: Observed: 250kDa

psi-iconProperties
PURIFICATION:Affinity purification
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS:Store at -20˚C. Avoid freeze / thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:POLR2A
ALTERNATE NAMES:POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
GENE ID:5430
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

ANTIBODIES FOR RESEARCH USE ONLY.

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