PIK3R4 Antibody Cat. No.: 7949

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.

The immunogen is located within amino acids 1290 - 1340 of PIK3R4.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: PIK3R4 antibody is human, mouse and rat reactive.
POSITIVE CONTROL:1) Cat. No. 1315 - Human Tonsil Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 149 kDa

Observed: 140 kDa

psi-iconProperties
PURIFICATION:PIK3R4 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PIK3R4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:PIK3R4
ALTERNATE NAMES:Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15
ACCESSION NO.:NP_055417
PROTEIN GI NO.:23943912
GENE ID:30849
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
REFERENCES:1) Herman PK, Stack JH, and Emr SD. A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J. 1991; 10:4049-60.
2) Stasyk OV, van der Klei IJ, Bellu AR, et al. A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy. Curr. Genet. 1999; 36:262-9.
3) Kihara A, Noda T, Ishihara N, et al. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J. Cell Biol. 2001; 152:519-30.
4) Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.

ANTIBODIES FOR RESEARCH USE ONLY.

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