Phosphotyrosine Antibody [PY20] Cat. No.: 33-259

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: All species
IMMUNOGEN: Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
TESTED APPLICATIONS: Flow, IF, IHC, IP, WB
APPLICATIONS: Flow Cytometry: 0.5-1 ug/million cells

IF: 1-2 ug/ml

WB: 0.5-1 ug/ml

IP: 1-2ug/500ug protein lysate

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.


psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG2b
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
GENE ID:Not Applicable
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

ANTIBODIES FOR RESEARCH USE ONLY.

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