Pellino 1 Antibody Cat. No.: 7071

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1.

The immunogen is located within amino acids 300 - 350 of Pellino 1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
 2) Cat. No. 17-011 - Hep G2 Cell Slide
PREDICTED MOLECULAR WEIGHT: 46 kDa

psi-iconProperties
PURIFICATION:Pellino 1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Pellino 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PELI1
ALTERNATE NAMES:Pellino 1 Antibody: PRISM, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-related intracellular-signaling molecule, Pellino-1
ACCESSION NO.:NP_065702
PROTEIN GI NO.:11037063
GENE ID:57162
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
REFERENCES:1) Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.
2) Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6
3) Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.
4) Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.